Originally part of the Sarasota Subdivision, this line was operated by the Seaboard Air Line before it came under ownership of CSX. The southern abandoned portions of the right-of-way between Sarasota and Venice were purchased by local governments from CSX in 2004 for a county-wide trail system, The Legacy Trail. The remainder of the right-of-way on the northern end, towards Oneco, was purchased by the Seminole Gulf Railway in 1987; the SGLR still serves some customers there, and has an interchange with CSX in Oneco.
Thanks to Neil Stutzman for contributing information about this route.